Journal: Drug Design, Development and Therapy

Article Title: Combination of Ginsenoside Rg1 and Astragaloside IV reduces oxidative stress and inhibits TGF-β1/Smads signaling cascade on renal fibrosis in rats with diabetic nephropathy

doi: 10.2147/DDDT.S171286

Figure Lengend Snippet: The primer sequences used for mRNA real-time PCR analysis

Article Snippet: Polyclonal antibodies used for immunohistochemical analysis included TGF-β1 (Santa Cruz Biotechnology, Dallas, TX, USA), Smad2/3 (Cell Sig-naling, Danvers, MA, China), Smad7 (Bioss, Woburn, MA, USA) and CTGF (Bioss).

Techniques: Real-time Polymerase Chain Reaction

Journal: Drug Design, Development and Therapy

Article Title: Combination of Ginsenoside Rg1 and Astragaloside IV reduces oxidative stress and inhibits TGF-β1/Smads signaling cascade on renal fibrosis in rats with diabetic nephropathy

doi: 10.2147/DDDT.S171286

Figure Lengend Snippet: The gray values of TGF-β1, Smad2/3, Smad7 and CTGF in groups. Notes: * P <0.05 vs N group; # P <0.05 vs M group; & P <0.05 vs G group; Ψ P <0.05 vs A group. The severer the immunopositivity was, the lower the gray value tested.

Article Snippet: Polyclonal antibodies used for immunohistochemical analysis included TGF-β1 (Santa Cruz Biotechnology, Dallas, TX, USA), Smad2/3 (Cell Sig-naling, Danvers, MA, China), Smad7 (Bioss, Woburn, MA, USA) and CTGF (Bioss).

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Combination of Ginsenoside Rg1 and Astragaloside IV reduces oxidative stress and inhibits TGF-β1/Smads signaling cascade on renal fibrosis in rats with diabetic nephropathy

doi: 10.2147/DDDT.S171286

Figure Lengend Snippet: The ratio of TGF-β1, Smad7 and CTGF mRNA expression in each group. Notes: * P <0.05 vs N group; # P <0.05 vs M group; & P <0.05 vs G group; Ψ P <0.05 vs A group.

Article Snippet: Polyclonal antibodies used for immunohistochemical analysis included TGF-β1 (Santa Cruz Biotechnology, Dallas, TX, USA), Smad2/3 (Cell Sig-naling, Danvers, MA, China), Smad7 (Bioss, Woburn, MA, USA) and CTGF (Bioss).

Techniques: Expressing

Journal: Cell

Article Title: Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease

doi: 10.1016/j.cell.2018.08.067

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SMAD7 , Atlas Antibodies , Cat#HPA028897; RRID: AB_10600811.

Techniques: Recombinant, Coagulation, Antibody Labeling, Membrane, Plasmid Preparation, Polymer, Blocking Assay, Staining, Imaging, cDNA Synthesis, RNAscope, DNA Library Preparation, Software, Hybridization

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of CPhGs on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p < 0.01 compared with control group, # p < 0.05, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of echinacoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p < 0.01 compared with control group, # p < 0.05, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of acteoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p < 0.01 compared with control group, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of CPhGs on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data are expressed as mean ± SD. ** p < 0.01 compared with control group, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Expressing, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of echinacoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p < 0.01 compared with control group, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Expressing, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Effects of acteoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p < 0.01 compared with control group, ## p < 0.01 compared with TGF-β 1 group.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Expressing, Control

Journal: Molecules

Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β 1 /smad as Potential Anti-Hepatic Fibrosis Agents

doi: 10.3390/molecules21010102

Figure Lengend Snippet: Primer sequences for RT-PCR.

Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

Techniques: Sequencing

Journal: Molecular Medicine Reports

Article Title: Effects of bradykinin on TGF-β1-induced epithelial-mesenchymal transition in ARPE-19 cells

doi: 10.3892/mmr.2018.8556

Figure Lengend Snippet: Primers used for reverse transcription-quantitative polymerase chain reaction amplification.

Article Snippet: The antibodies and dilution ratios were as follows: Anti-E-cadherin (1:500; cat. no. GTX100443, GeneTex International Corporation, Hsinchu, Taiwan), mouse anti-α-SMA (1:500; cat. no. BS70000, Bioworld Technology, Inc., St. Louis Park, MN, USA), vimentin (cat. no. BS1491, 1:500; Bioworld Technology, Inc.), anti-pSmad3 (1:500; cat. no. BS4173, Bioworld Technology, Inc.), anti-Smad7 (1:500; cat. no. BS60366, Bioworld Technology, Inc.) and anti-GAPDH (1:500; cat. no. G9545, Sigma-Aldrich; Merck KGaA).

Techniques: Polymerase Chain Reaction, Amplification

Journal: Molecular Medicine Reports

Article Title: Effects of bradykinin on TGF-β1-induced epithelial-mesenchymal transition in ARPE-19 cells

doi: 10.3892/mmr.2018.8556

Figure Lengend Snippet: BK inhibits TGF-β1-induced epithelial-mesenchymal transition via the TGF/Smad signaling pathway. ARPE-19 cells were treated with BK, TGF-β1, BK plus TGF-β1, TGF-β1 plus HOE140, BK plus TGF-β1 plus HOE140 and Dulbecco's modified Eagle's medium for 48 h before detection. (A) Protein expression of pSmad3 and Smad7 was identified by western blot analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis and (C) immunofluorescence staining (magnification, ×200). **P<0.001 and *P<0.05. TGF-β1, transforming growth factor-β1; BK, bradykinin; pSmad, phosphorylated mothers against decapentaplegic homolog.

Article Snippet: The antibodies and dilution ratios were as follows: Anti-E-cadherin (1:500; cat. no. GTX100443, GeneTex International Corporation, Hsinchu, Taiwan), mouse anti-α-SMA (1:500; cat. no. BS70000, Bioworld Technology, Inc., St. Louis Park, MN, USA), vimentin (cat. no. BS1491, 1:500; Bioworld Technology, Inc.), anti-pSmad3 (1:500; cat. no. BS4173, Bioworld Technology, Inc.), anti-Smad7 (1:500; cat. no. BS60366, Bioworld Technology, Inc.) and anti-GAPDH (1:500; cat. no. G9545, Sigma-Aldrich; Merck KGaA).

Techniques: Modification, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

Journal: Journal of Biological Chemistry

Article Title: Vascular Endothelial Cell Growth Factor Attenuates Actions of Transforming Growth Factor-β in Human Endothelial Cells

doi: 10.1074/jbc.m407423200

Figure Lengend Snippet: FIG. 7. Effect of VEGF on binding of Smad7 to TGF- receptor type I in HUVEC. HUVEC cells were incubated with 30 nM VEGF for 60 min, and the cell lysates were immunoprecipitated (IP) by Smad7 antibody (aSmad7) and immuno- blotted (IB) with antibodies against TGF- receptor type I (aTGFRI) or aSmad7. These data are representative of three independent experiments. The den- sitometry data are presented as relative density compared with the density at the basal (untreated) condition. **, p 0.01 versus basal expression.

Article Snippet: Anti-Smad2/3 rabbit polyclonal antibody (aSmad2/3), anti-phospho-Ser465/467-specific Smad2/3 rabbit polyclonal antibody (apSmad2/3), anti-Smad7 rabbit polyclonal antibody (aSmad7), and TGF- receptor type I (ALK5) mouse monoclonal antibody (aTGFRI) were obtained from Cell Signaling Technology (Beverly, MA), Santa Cruz Biotechnology (Santa Cruz, CA), Upstate Biotechnology (Lake Placid, NY), and BD Biosciences, respectively.

Techniques: Binding Assay, Incubation, Immunoprecipitation, Expressing